Dušica Kovačević, Katarina Zečević, Ivana Stanković
Glasilo Future Volumen 7 broj 4 listopad 2024. / Gazette Future Volume 7 number 4 October 2024
Objavljeno 09. listopada 2024. godine. / Published on October 09, 2024.
https://doi.org/10.32779/gf.7.4.1
ISSN 2623-6575 (Online)
UDK 63
Glasilo_Future_Volumen_7_broj_4_listopad_2024.pdf
Izvorni znanstveni rad (original scientific paper)
Naslov/Title
Partial molecular characterization of cucumber mosaic virus isolate infecting garden peony (Paeonia officinalis) in Serbia
Str./Pp. 01–13
Autori(ce)/Authors
Dušica Kovačević, Katarina Zečević, Ivana Stanković
Abstract
Paeonia officinalis (family Paeoniaceae), known as the garden peony, is a very popular flowering plant with large, showy flowers grown in many gardens in Serbia. In May 2021, peony plants showing chlorotic ringspot and severe mosaic of leaves were observed in a private garden in Zemun (District of Belgrade, Serbia). Symptomatic leaves from two plants were collected and analysed for the presence of cucumber mosaic virus (CMV), tobacco rattle virus (TRV), alfalfa mosaic virus (AMV) and tomato spotted wilt virus (TSWV) using commercial ELISA kits. CMV was detected serologically in both peony samples and no other plant virus was identified. The causal agents from both ELISA-positive samples were successfully mechanically transmissible to Chenopodium quinoa and Nicotiana glutinosa plants. CMV infection in symptomatic garden peony plants was also confirmed by RT-PCR with CMV-specific primers amplifying the complete coat protein (CP) gene and parts of the 3′- and 5′-UTRs. Selected ELISA-positive sample (318-21) was Sanger sequenced using the same primer as in RT-PCR and phylogenetic tree based on complete CP sequences showed that Serbian CMV isolate from garden peony belongs to the CMV subgroup IA.
Key words: peony, viruses, Cucumovirus, ELISA, bioassay, RT-PCR, phylogenetic analysis.
Preuzimanje/Download
Citiranje/Citation
Kovačević, D., Zečević, K., Stanković, I. (2024). Partial molecular characterization of cucumber mosaic virus isolate infecting garden peony (Paeonia officinalis) in Serbia. Glasilo Future, 7(4), 01–13.